Using high-throughput sequencing to leverage surveillance of genetic diversity and oseltamivir resistance: a pilot study during the 2009 influenza A(H1N1) pandemic.
BACKGROUND: Influenza viruses display a high mutation rate and complex evolutionary patterns.Next-generation sequencing (NGS) has been widely used for qualitative and semi-quantitative assessment of genetic diversity in complex biological samples.The "deep sequencing" approach, enabled by the enormous throughput of current NGS platforms, allows the